This report gives an overview of the most important findings of SynthEthics second work package, “Ethical and regulatory challenges raised by synthetic biology”. Our partner in Padua prepared an elaborate overview of all relevant areas in law that were identified for synthetic biology. It includes the following regulatory areas: issues connected to genetically modified organisms, synthetic biology and biofuels, synthetic biology and biomedical applications, applications of synthetic biology in cosmetics, issues of intellectual property, bio-Informatics, synthetic biology and occupational health, issues connected to human rights, issues connected to precaution, the role of soft law and the convergence of ethical and legal principles into (mainly soft) regulatory tools. On the basis of input provided by our partner in Padua, our partner in Delft held a survey amongst scientists to gain insight in the knowledge, views and opinions of expert scientists working in synthetic biology and related fields. All questions raised in this context were further elaborated through interviews with experts in policy making on regulating synthetic biology.